Journal of Science, S. 1 (2016)

Cỡ chữ:  Nhỏ  Vừa  Lớn

DNA hypermethylation patterns of APC gene promoter in Vietnamese High-Risk HPV infected patients

TRUONG KIM PHUONG, LAO DUC THUAN, LE HUYEN AI THUY

Tóm tắt


    Cervical cancer is the leading cause of cancer death in women in Vietnam. Virtually,cervical cancers are associated with infection of HPV (Human papilloma virus). In addition,inactivation of tumor suppressor genes (TSGs), leading by aberrant hypermethylation, anepigenetic mechanism, has been observed in cervical cancer development. Screening for earlydetection of cervical cancer is importantly increasing from Vietnam, therefore, in current study,we analyzed the aberrant methylation status of APC (Adenomatous polyposis coli) gene, itsproduct has an important role in cell cycle control and maintenance of genomic stability, as thepattern of potential biomarker for cervical cancer in Vietnamese population. The liquid-basedPap test samples which were identified whether HPV-infected or low-risk HPV infected or non-HPV-infected were enrolled and analyzed by MSP (Methylation specific PCR). As the results, thehypermethylation of APC was reached to 75%, 12.5% and 30% in high-risk HPV genotypeinfected group, in low-risk HPV genotype infected group, and non-HPV genotype infection,respectively. Especially, the characteristic of high-risk HPV infection was also associated withthe hypermethylation of candidate gene (p < 0.05). Moreover, the odds ratio and relative riskwere found in the high value, counting for 10.5 (95%CI, 2.3 - 47.2) and 3.37 (95%CI, 1.3 - 8.3),respectively. In conclusion, these outcomes suggested that the aberrant hypermethylation of APCgene, which accessed in non-invasive samples, led to the potential biomarker and application inearly prognosis and diagnosis to cervical cancer in Vietnamese population.

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Journal of Science - Ho Chi Minh City Open University